ProteOn Label-Free webinars now available on YouTube.

We hope you enjoyed attending some or all of our webinars. You may access all the recorded material using the link below. This link also allows you to access our extensive webinar library.

ProteOn YouTube Library »

Past Webinar Details

Screening and selection of anti-Clostridium monoclonal antibodies using the ProteOn™ XPR36 system: developing a diagnostic test for Clostridium

Presenter: Dr Carlotta Chiappa, Research Scientist, DiaSorin Research Center, Italy

Clostridium difficile infections are increasing in incidence, severity, and mortality: rapid identification of virulent strains is essential to preventing their spread. In this webinar, Dr Carlotta Chiappa will present how the ProteOn XPR36 system played a key role in screening and selecting specific MAbs for the development of a chemiluminescent immunoassay to detect C. difficile antigens. The flexibility in assay orientation options provided by the ProteOn system allowed multiple analyses on hybridoma supernatants to identify the best candidates in terms of affinity and performance prediction.

Best practices in data processing and analysis to achieve high-quality SPR results

Presenter: Dr Jonathan Popplewell, Field Application Scientist, Bio-Rad Laboratories, UK

This webinar will focus on the best practices for data processing and analysis in surface plasmon resonance (SPR) experiments. As a senior field application scientist working on the ProteOn™ XPR36 system, Dr Jonathan Popplewell has over 10 years experience of label-free analysis. He will cover a range of topics including the use of standard errors, use of local versus grouped / global fits, generating standard deviations, and how to put data sets together for statistical analysis. Attendees will gain deep knowledge of the many factors included in data processing and analysis, as well as how to design experiments for better data acquisition. These best practices will lead to high-quality SPR results.

Using the ProteOn™ XPR36 system to characterize human-derived monoclonal antibodies against influenza virus

Presenter: Dr Lisa Scalfone, Postdoctoral Fellow, Stanford University School of Medicine, USA

Different strains of Influenza virus include a great diversity of antigenic proteins, often requiring annual vaccination for strain-specific protection. This webinar will focus on the use of the ProteOn XPR36 system to determine the cross-reactivity and binding kinetics of a panel of human-derived monoclonal antibodies against the H3 hemagglutinin protein in multiple Influenza strains. The ability to robustly measure the binding affinities of a panel of antibodies with numerous antigens using the ProteOn system offers a powerful platform that is applicable to many experimental systems. It allows real-time comparison of multiple interactions on a single chip and provides high throughput in obtaining the screening result.

Novel applications of ProteOn™ XPR36 system in protein-protein, lipid-protein, and DNA-protein interaction analysis

Presenter: Ladislav Bumba, Institute of Microbiology, Czech Academy of Sciences, Czech Republic

Surface Plasmon Resonance (SPR) is employed as a technology to measure biomolecular interactions in real-time in a label-free manner. Using this method, one of the interacting partners (ligand) is immobilized to the sensor surface, while the other (analyte) is free in solution and passed over the sensor’s surface. The kinetic parameters of the interactions are further calculated from association and dissociation sensorgrams. This lecture will introduce an SPR optical biosensor ProteOn XPR36 system which features a 6 x 6 interaction array for the simultaneous analysis of up to six ligands with up to six analytes which enables analysis of up to 36 different protein interactions in a single run on a single chip. The protein-protein interaction of adenylate cyclase toxin (CyaA) with integrin receptor CD11b/CD18, lipid-protein interaction of phosphatidylinositol 4,5-bisphosphate (PIP2) with membrane channel TrpV1, and DNA-protein interaction of transcription factor FoxO4 with target DNA, will be presented to show the throughput, flexibility and versatility of this instrument.



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